Active site remodeling in tumor-relevant IDH1 mutants drives distinct kinetic features and potential resistance mechanisms

Mutations in human isocitrate dehydrogenase 1 (IDH1) drive tumor formation in a variety of cancers by replacing its conventional activity with a neomorphic activity that generates an oncometabolite. Little is understood of the mechanistic differences among tumor-driving IDH1 mutants. We previously reported that the R132Q mutant uniquely preserves conventional activity while catalyzing robust oncometabolite production, allowing an opportunity to compare these reaction mechanisms within a single active site. Here, we employed static and dynamic structural methods and found that, compared to R132H, the R132Q active site adopted a conformation primed for catalysis with optimized substrate binding and hydride transfer to drive improved conventional and neomorphic activity over R132H. This active site remodeling revealed a possible mechanism of resistance to selective mutant IDH1 therapeutic inhibitors. This work enhances our understanding of fundamental IDH1 mechanisms while pinpointing regions for improving inhibitor selectivity.

While early kinetic characterization of IDH focused on bacterial forms, recent efforts have illuminated details of human IDH1.As IDH1 WT binds its substrates, a conformational change occurs where the large domain (residues 1-103 and 286-414) and small domain (residues 104-136 and 186-285) move towards each other relative to a hinge point (residues 134-141) in the clasp domain (residues 137-185) 13 .This movement closes the active site cleft with a concomitant opening of a back cleft 13 .A critical regulatory segment comprised of the α10 helix (residues 271-285) helps stabilize an open, inactive conformation 13 in the absence of substrates, and undergoes a conformational change to help properly orient the active site residues upon substrate binding-driven closure 13 .These structural features are generally preserved in IDH1 R132H 1,3,14 , but inherent catalytic deficiencies coupled with improved NADPH binding allow this mutant to catalyze inefficient D2HG production, albeit at great benefit to the tumor environment.
To better understand how D2HG production occurs, there is tremendous value in studying a mutant with more robust neomorphic reaction catalytic efficiency.IDH1 R132S/L/G/Q mutations have also been reported in patients with various frequencies [15][16][17][18][19] , and different mutations have been shown to support distinct D2HG levels in tumors 20 .We have shown that these mutants have unique kinetic properties for both neomorphic and conventional reactions 21,22 , suggesting that their kinetic features may drive some of the variability in patients' D2HG levels 22 .We identified one mutant, R132Q, that uniquely maintained WT-like properties with modest conventional catalytic activity, but also drove unusually robust D2HG production 21 and was resistant to mutant IDH1 inhibitors via a mechanism not yet understood 22 .This R132Q mutant has been shown to drive enchondroma tumor formation in mouse models 23 .By establishing unique features of IDH1 R132Q and R132H mutants, we can identify additional selectivity handles for improved mutant IDH1 inhibitors, as an H-to-Q mutation requires only a single base change.Investigating the atomic-level mechanisms that drive such diverse kinetic activity and inhibition among tumor-relevant IDH1 mutants can also inform the types of chemical features that can guide the field of enzyme design 24 .
Here, we establish the static and dynamic structural features that drive the unique kinetic properties among tumor-relevant IDH1 mutants, capitalizing on the unusual active site attributes that allow IDH1 R132Q to maintain both normal and neomorphic activities.We identified multiple conformations for R132Q binding to the neomorphic substrate αKG, but not the conventional substrate ICT.Our kinetics and dynamic structural methods clarified that R132Q's ability to explore multiple conformations and substrate binding modes occurred within a relatively immobile, solvent-inaccessible enzyme that is better optimized for substrate binding, hydride transfer, and mutant IDH1 inhibitor resistance as compared to R132H.

Results
Kinetic features of IDH1 R132Q suggest structurally optimized substrate binding and hydride transfer steps relative to R132H.
We previously demonstrated that IDH1 R132Q uniquely maintains modest catalytic efficiency for the conventional reaction (ICT to αKG conversion), while also displaying much higher catalytic efficiency for the neomorphic reaction (αKG to D2HG conversion) relative to R132H 21,22 .Steady-state kinetics analysis performed for this present study (Extended Data Fig. 1) revealed a 5.6-fold increase in catalytic efficiency for the conventional reaction in IDH1 R132Q versus R132H, driven primarily by an increase in kcat.R132Q catalyzed the neomorphic reaction 9.4-fold more efficiently than R132H via an increase in kcat and decrease in Km.This suggests that IDH1 R132Q exhibits a more stable transition state and provides more optimized on/off paths of the reactants and products compared to R132H.
Pre-steady-state kinetics experiments indicated that hydride transfer, or a step preceding it, was ratelimiting for the conventional reaction catalyzed by WT and R132Q, and for the neomorphic reaction catalyzed by R132Q and R132H (Fig. 1).Interestingly, NADPH consumption by R132H showed an initial lag that could be eliminated upon using higher concentrations of αKG (Extended Data Fig. 2).A lag has been reported previously with IDH1 WT, which was eliminated via pre-incubation of both ICT and metal [25][26][27][28] .Interestingly, we did not observe a lag in the neomorphic reaction catalyzed by R132Q, despite using a concentration of αKG that was 10-fold lower than the concentration associated with a lag in R132H.This suggests that αKG is more proficient at driving IDH1 R132Q from an inactive to an active state compared to R132H, though it was not apparent through these experiments whether this was achieved by a more catalytically primed ground state or a faster conformational change.We were unable to capture rates of conformational change when monitoring intrinsic protein fluorescence.However, we measured rates of NADPH binding to IDH1 WT, R132H, and R132Q using enzyme that was stripped of cofactor 14 (Extended Data Fig. 3).We found that all three IDH1 proteins displayed singlestep binding events, with an NADPH binding on rate (kon) for IDH1 WT that was ~2-fold faster than that calculated for IDH1 R132Q, while kon rates for IDH1 R132H were profoundly slower.We also used isothermal titration calorimetry (ITC) to measure equilibrium binding affinity of NADPH for IDH1 (Supplementary Fig. 1).
We found that both mutants exhibited a 5-fold decrease in Kd compared to IDH1 WT, suggesting that a slower koff rate drove the improved affinity for NADPH observed for R132H despite the slow kon rate.Taken together, these kinetic data further supported the finding that when compared to R132H, IDH1 R132Q has a lower barrier to adopting the closed, active conformation that is driven by substrate and metal binding.

IDH1 R132Q has a less solvent accessible active site pocket that is more catalytically primed.
To illuminate possible mechanisms behind the time-resolved changes exhibited by IDH1 R132Q versus those in WT and R132H, we first used hydrogen/deuterium exchange-mass spectrometry (HDX-MS) analysis.
We probed solvent accessibility as indicated by deuterium uptake in the binary IDH1:NADP(H) form, as WT and mutant IDH1 are known to copurify bound to NADP(H) 26,27 .We also measured deuterium uptake upon the addition of substrate (ternary complex, IDH1:NADP(H):ICT/αKG), or upon the addition of substrate and Ca 2+ (quaternary complex, IDH1:NADP(H):ICT/αKG:Ca 2+ ).By far the most substantial change in deuterium uptake for WT, R132H, and R132Q occurred in the quaternary form, indicative of closed, catalytically competent conformations among all enzyme species (Extended Data Fig. 4).This is consistent with previous findings that both substrate (ICT, but also presumably αKG in the neomorphic reaction) and divalent metal binding are required to drive IDH1 into its fully closed, active conformation [25][26][27][28] .Deuterium uptake generally showed the following trend: R132H:NADPH:αKG:Ca 2+ >> WT:NADP + :ICT:Ca 2+ > R132Q:NADPH:ICT:Ca 2+ > R132Q:NADPH:αKG:Ca 2+ (Fig. 2, Extended Data Fig. 5), with R132Q appearing to have an overall less structurally dynamic, more closed conformation compared to R132H.Since our kinetic studies suggested IDH1 R132Q had a lower barrier to achieve the closed conformation compared to R132H, we hypothesized that the binary R132Q:NADP(H) would be in a more quaternary-like state.To test this, we compared deuterium uptake among the binary states, predicting that the R132Q:NADP(H) complex would experience less deuterium uptake than R132H:NADP(H).Unsurprisingly, in general the IDH1:NADP(H) forms of all three proteins had high deuterium uptake, particularly in the substrate binding pocket, clasp domain, and dimer interface (Fig. 2, Extended Data Figs.4, 5).As predicted, R132Q:NADP(H) and WT:NADP(H) had the least deuterium uptake overall, while R132H:NADP(H) exhibited, by far, the most uptake.As this suggested that NADP(H)-bound R132Q had a more closed/less mobile conformation compared to R132H, we wondered if the temporal features of our HDX-MS data suggested a faster closing upon substrate binding for the R132Q mutant.This would provide one mechanism of the improved catalytic efficiency shown by IDH1 R132Q relative to R132H in both the conventional and neomorphic reactions.To address this, we inspected peptides that included residues within 4 Å of the bound NADP(H) and ICT/αKG substrates to determine if deuterium uptake equilibrium was reached faster in IDH1 R132Q versus R132H, consistent with a primed ground state that reached a closed conformation more easily.
Interestingly, the peptides that contained active site residues from the adjacent monomer (i.e., chain B residues contributing to the chain A active site) showed a faster approach to deuterium uptake equilibration for IDH1 R132Q and WT compared to R132H (Extended Data Fig. 6).Specifically, peptides 210-216, 240-253, and 257-267 all showed IDH1 R132Q reaching an equilibrium state faster than R132H.This is supportive of a model where the ground state of R132Q is a more closed conformation that follows a simpler path to a catalytically competent state compared to R132H.
Binary structures of IDH1 R132Q were valuable to help us understand how the active site of cofactorbound R132Q compared to R132H.While R132Q:NADP(H) showed no major global structural alterations upon alignment with previously solved structures of WT:NADP(H) 13 and R132H:NADP(H) 29 , many local shifts were observed (Fig. 3).Unsurprisingly, NADP(H)-bound R132Q had the typical overall open, inactive conformation seen in WT and R132H, with a larger active site cleft and smaller back cleft relative to the quaternary complexes (Supplementary Table 1).Interestingly, these distances in the binary IDH1 R132Q structure more closely resembled binary WT than R132H, supportive of a more closed, catalytically competent ground state for R132Q.However, IDH1 R132Q exhibited notable differences compared to WT and R132H.In particular, the clasp domain and helices proximal to the substrate and cofactor binding site were shifted, with the α1, α2, α4, α5, and α11 helices adjusted upwards and inwards in R132Q versus WT and R132H binary complexes, resulting in a similar shift of the NADP(H) molecule itself (Fig. 3B).Importantly, this inward shifting of the α1 helix is a feature of closed, catalytically competent IDH1 conformations.Notably, R132Q also contained longer, more intact β strands in the clasp domain, which plays a major role in maintaining the dimer, compared to both WT and R132H (Fig. 3).The fully intact β7 and β8 strands in R132Q were reminiscent of quaternary, fully substrate-bound forms of IDH1 WT and R132Q (vide infra).Consistent with such stable secondary structure, peptides in the β8 strand of R132Q:NADP(H) had lower deuterium uptake than WT:NADP(H) and R132H:NADP(H) (Fig. 2, Extended Data Fig. 5).IDH1 R132Q also maintained an extensive hydrogen bonding network enveloping the NADP(H) molecule; this network was far less robust in R132H (Extended Data Fig. 7).
Together, both dynamic and static structural data suggest that the IDH1 R132Q active site pocket and surrounding features have greater rigidity and more defined structural features typical of fully-substrate-bound forms of IDH1, suggesting a more catalytically primed state for R132Q:NADP(H) compared to R132H:NADP(H).WT:NADP(H) 13 , and R132H:NADP(H) 29 .C) Monomer-based alignments of R132Q:NADP(H), WT:NADP(H) 13 , and R132H:NADP(H) 29 .The inset features catalytic residues Y139 and K212 (though the latter residue drives catalysis in the monomer not shown), residue R132(H/Q), and the cofactor.

Unlike R132H, ICT-bound IDH1 R132Q is in a closed, catalytically competent conformation.
Here, we also report the first ICT-bound quaternary structure of IDH1 R132Q (R132Q:NADP(H):ICT:Ca 2+ ).Upon alignment of this structure with WT:NADP(H):ICT:Ca 2+ 13 (Fig. 4), there was obvious overlap in both global features and active site details.ICT-bound IDH1 R132Q also aligned well with R132H bound to its preferred substrate, αKG (R132H:NADP(H):αKG:Ca 2+ ) 14 .Like ICT-bound WT and αKGbound R132H structures, ICT-bound IDH1 R132Q adopted a catalytically competent, closed conformation, with ICT maintaining many of the same polar interactions with the protein and divalent ion as observed with IDH1 WT.This is supportive of our kinetic data showing R132Q's preservation of the conventional activity.Though alignment of ICT-bound WT and R132Q was strikingly similar, the notable 180-fold decrease in catalytic efficiency suggested that maintaining hydrogen bonding features and active site structuring was not sufficient for robust conventional activity in R132Q.Interestingly, ICT was observed only in one monomer of the R132Q quaternary complex, resulting in a shift of the α11 helix and the NADP(H) molecule upward and outward in the ICT-absent R132Q monomer (Fig. 4), reminiscent of the WT:NADP(H) binary structure (Fig. 3).This lack of active site saturation suggested a lower affinity toward ICT for IDH1 R132Q versus WT.Though Km values are not affinity measurements, it is noteworthy that there was a >300-fold increase in Km when comparing R132Q to WT.To address differences in binding affinity, we again turned to ITC experiments.While ICT binding affinity for IDH1 R132H was below the limit of detection, we found that R132Q exhibited a ~170fold decrease in ICT affinity compared to WT (Supplementary Fig. 1).Further, evidence of ICT binding was observed for R132Q, but not R132H.Structural studies again provided a possible mechanism; in contrast to the closed, catalytically competent conformation of ICT-bound R132Q, a previously solved ternary R132H:NADP(H):ICT 29 structure revealed quasi-open monomers that had α4 and α11 helices shifted upwards and outwards from the dimer interface and an unraveled α10 helix (Fig. 4B, 4C), regions we and others have shown to be highly flexible 13,[29][30][31] .Notably, ICT was found in a posited pre-binding site that was shifted to the left of its catalytically-competent position 29 .This resulted in limited polar interactions by ICT to R132H 29 in contrast to ICT's extensive polar contacts to R132Q, including hydrogen bonding to catalytic residue Y139 that indicated a catalytically-ready binding conformation (Extended Data Fig. 7).As further evidence that ICT-bound R132H was ill-prepared for catalysis, its catalytic residues swung away from the active site, akin to the positioning found in binary, catalytically incompetent IDH1 structures (Fig. 4C).Though this IDH1 R132H structure did not include a divalent metal that may be required for full closure 29 , it is nonetheless unsurprising that IDH1 R132H, in contrast to IDH1 R132Q, is essentially unable to convert ICT to αKG.

IDH1 R132Q αKG-bound form is semi-closed, with an αKG binding pocket that is unique from R132H.
Since IDH1 R132Q uniquely maintains both normal and neomorphic catalytic abilities, we asked how the binding conformations for ICT, the conventional reaction substrate, and αKG, the neomorphic reaction substrate, compared.Here, we report two αKG-containing IDH1 R132Q quaternary structures (R132Q:NADP(H):αKG:Ca 2+ ).These co-crystallization experiments led to a variety of complexes, with monomer asymmetry still observed (Fig. 5).One structure had αKG bound in one monomer, and a covalent NADP-αKG adduct in the other (Fig. 5A, Supplementary Fig. 2).Cleft measurements in both monomers indicated a slightly more open conformation when compared to the closed quaternary R132Q (ICT-bound), WT (ICT-bound) and R132H (αKG-bound) structures, with the α11 helix shifted out away slightly from the substrate binding pocket (Fig. 5E).As a result, the NADP(H) itself shifted outwards compared to the ICT-bound R132Q structure, resulting in a semi-closed conformation (Supplementary Table 1).The second αKG-bound structure had unique features among two dimers in the crystallographic asymmetric unit.One catalytic dimer contained one NADP-αKG adduct and one αKG molecule (Fig. 5B), and again appeared as an intermediate between the R132Q:NADP(H) and the R132Q:NADP(H):ICT:Ca 2+ structures (Supplementary Table 1).A second dimer contained an NADP-αKG adduct in one monomer, while no αKG-containing molecule was observed in the adjacent monomer (Fig. 5C).This dimer was in a more closed, catalytically competent conformation, reminiscent of the fully closed WT quaternary structure (Supplementary Table 1).The Ca 2+ ion clearly led to extensive restructuring, as the R132Q:NADP(H):Ca 2+ monomer aligned relatively poorly with the R132Q:NADP(H) complex despite the only difference being the presence of the metal ion (Fig. 5H).Thus, closing of IDH1 R132Q to the αKG-bound form may be driven just as much by metal binding as by substrate binding.This finding was recapitulated by the overall decrease seen in deuterium uptake upon treatment of substrate-bound R132Q with Ca 2+ (Extended Data Fig. 4).Overall, we were able to capture snapshots of stable conformations of αKG binding ranging from semi-closed (αKG-bound) to essentially fully closed (NADP-αKG adduct-bound).
Closed conformations are seen for WT 13 and R132H 14 when bound with their preferred substrates (ICT and αKG, respectively).As αKG-bound R132Q was often not as fully closed as the ICT-bound form, we wondered how αKG-bound R132Q compared to these WT and R132H closed conformations.In alignments of R132Q:NADP(H):αKG:Ca 2+ with quaternary WT and R132H structures (Fig. 5D, 5E), the catalytic residue Y139 in R132Q was shifted away from the αKG molecule, with this molecule making fewer hydrogen bond contacts within the R132Q active site compared to R132H (Extended Data Fig. 7).In R132Q, the αKG binding site was shifted upwards towards NADP(H) and away from the substrate binding sites seen in the ICT-bound WT and αKG-bound R132H structures.This shift might be facilitated by one surprising feature of all non-αKGcontaining R132Q monomers --the nicotinamide ring could not be reliably modeled due to missing electron density (Fig. 5).This suggests that when αKG was absent (such as in the R132Q:NADPH:Ca 2+ monomer that dimerized with the NADPH-αKG adduct) or, more unexpectedly, even when αKG was bound (R132Q:NADPH:αKG:Ca 2+ monomers), this portion of NADP(H) was more dynamic in the active site.Overall, the αKG-containing R132Q structures either did not appear in a catalytically-ready form, or the enzymatic mechanism may rely more heavily on different amino acids used in the conventional reaction.

ICT-bound and αKG-bound IDH1 R132Q show unique static and dynamic structural features.
The α10 regulatory segment undergoes notable restructuring upon substrate binding, with this segment forming a helix in both the ICT-and αKG-bound quaternary forms of R132Q, just like in ICT-bound WT and αKG-bound R132H (Figs. 4, 5).However, our HDX-MS experiments captured more subtle differences in R132Q that depended on the substrate that was bound.The α10 regulatory segment and the nearby α9 helix were much more protected from proton exchange in both αKG and αKG + Ca 2+ conditions in R132Q than in the ICT and ICT + Ca 2+ conditions (Figs. 2, 6).Beyond its proximity to the regulatory segment, the α9 helix has an additional role in active site remodeling in that it helps form a "seatbelt" that envelopes the NADP(H) cofactor in many aldo-keto reductase enzymes (reviewed in 32 ).This seatbelt was observed in the WT:NADP(H):ICT:Ca 2+ quaternary structure, with residue R314 in α11 helix shifted inward to form polar contacts with D253' and Q256' in α9 of the adjacent monomer and with a water molecule (Fig. 7).The absence of the seatbelt was not limited to binary R132Q, R132H, and WT structures; no seatbelt was observed in the ternary ICT-bound or, more surprisingly, in the closed, quaternary αKG-bound R132H structures 14,29 .As no αKG-bound IDH1 WT structure is available at this time, we compared a structure of a non-R132 mutant, G97D, which generates D2HG but exhibits a high degree of structural similarities with IDH1 WT 14 .The αKG-bound form of G97D also did not show a seatbelt conformation, suggesting this is a unique feature of ICT-bound, fully closed structures.IDH1 R132Q behaved like WT when binding the conventional reaction substrate (ICT), with a seatbelt forming over the cofactor since residue R314 was in position to contact Q256', D253', and, unique to this protein, E247' in β11 of the adjacent monomer, as well as a water molecule (Fig. 7).However, R132Q behaved more like R132H when binding the neomorphic substrate, with αKG-bound monomers showing residue R314 swung away from the α9' helix, precluding the necessary polar contacts.Interestingly, the closed R132Q:NADP-αKG:Ca 2+ /R132Q:NADP(H):Ca 2+ dimer (Fig. 5C) had an intact seatbelt over the NADP-αKG adduct (Fig. 7B), suggesting that a fully closed conformation of αKG-bound IDH1 R132Q is possible if the nicotinamide ring of NADP(H) is stabilized in some way, such as via adduct formation.Interestingly, HDX-MS dynamics showed that seatbelt formation was associated with an increase in deuterium uptake, with the α11 helix, which contains the seatbelt-forming R314 residue, more protected in the αKG-bound R132Q and R132H (seatbelt-lacking) complexes relative to the ICT-bound WT and R132Q (seatbelt-forming) complexes (Fig. 6).
Overall, multiple conformations are possible with αKG-containing R132Q structures, including those associated with fully closed forms.In addition to the NADP-αKG adduct, we also encountered an NADP-tris(2-carboxyethyl)phosphine (NADP-TCEP) adduct when attempting to crystallize ICT-bound R132Q (Fig 5F, Supplementary Fig 2).There appeared to be some catalytic relevance of these adducts in that the TCEP and αKG carboxylates in the adducts helped coordinate the Ca 2+ and maintained many hydrogen bonds in their respective active sites, though the metal ion was slightly shifted to accommodate the adducts (Fig. 5, Supplementary Fig. 4).All TCEP and αKG adducts appeared as hybrids between the semi-closed αKG-bound R132Q complex and fully closed ICT-bound R132Q complex (Supplementary Table 1).In general, One NADP-αKG adduct-containing monomer (Fig. 5C) aligned well to the fully closed ICT-bound R132Q structure in all regions except the clasp domain, where the adducted monomer was shifted towards the dimer interface and the β9 strand was more intact (Fig. 5G).As further evidence of its fully closed conformation, this NADP-αKG adduct-containing monomer also had an intact seatbelt feature (Fig. 7B).
To better understand how these adducts were forming, we performed density functional theory (DFT) calculations for model NADP-TCEP and NADP-αKG adducts (Supplementary Tables 2, 3), which suggested that adduct formation would fail to occur if not for the constraining environment of the crystal structure.We considered an alternative possibility that the IDH1 R132Q active site itself favored adduct formation and binding.If the NADP-TCEP adduct could form in the active site of R132Q, it would act as a competitive inhibitor.Thus, we treated R132Q with varying concentrations of three reducing agents (TCEP, dithiothreitol (DTT), and β-mercaptoethanol (BME)) to determine the effects of catalysis of the conventional reaction (Extended Data Fig. 8, Supplementary Table 4).Dose-dependent inhibition of R132Q catalysis was profound with TCEP, while DTT and DME had minimal effects.More modest, though notable effects on catalysis were also observed when challenging IDH1 WT with the highest concentration of TCEP tested (10 mM) (Supplementary Fig. 3).As NADP-DTT adducts have been previously reported 33 , our discovery that DTT did not inhibit R132Q may help support a model where the enzyme supports adduct formation.While DTT has some similar structural features compared to ICT, it does not recapitulate the carboxylic acid features that TCEP and αKG provide in their NADP-containing adducts.Together, these results strongly support the hypothesis that adduct formation occurs outside of the non-physiologically-relevant crystal packing environment, with the adducts mimicking αKG binding, ICT binding, or transition between the two.
As these adduct-containing structures showed hybrid binding features of αKG and ICT, we wondered if transition state features could be extrapolated.Here, the nicotinamide ring of the adduct lent an interesting clue.Calculations suggest that the nicotinamide ring is likely to be planar in the oxidized form 34,35 .During NADP + activation for hydride transfer, the enzyme is predicted to distort the nicotinamide ring to form a highly puckered transition state as a partial positive charge on C4N develops [34][35][36] (Supplementary Fig. 5).NAD(P)adducts with reducing agents have been reported previously, including with TCEP 37 and also with DTT 33 , and were often found to have a more puckered nicotinamide ring, reminiscent of a transition state.Here, unlike the planar ring observed in our non-adducted forms of NADP(H) (R132Q:NADP(H):ICT:Ca 2+ ), both the αKG-and TCEP-containing NADP adducts showed a more puckered nicotinamide ring (Supplementary Fig. 4, Supplementary Table 3), suggestive of a transition-state-like conformation.
In summary, we highlight discrete catalytic and structural features among two tumor-relevant IDH1 mutants, with the IDH1 R132Q mutant serving as an invaluable tool to probe the journey through substrate turnover of two reactions that typically cannot be performed by the same enzyme.Together, our kinetics experiments and static and dynamic structural data suggests that substrate binding and conformational changes associated with the conventional and the neomorphic reactions have unique paths through turnover that can be described in terms of differences in substrate affinity, substrate binding site location, solvent accessibility, and propensity for conformational activation and active site remodeling (summarized in Fig. 8).IDH1 R132Q's accommodation of catalytically-relevant adducts, perhaps due to its active site appearing better optimized for catalysis compared to R132H, illuminate snapshots of substrate and substrate analogs in varying degrees of catalytic readiness.Helices displaying profound differences in alignment of the three forms of IDH1 are highlighted.The seatbelt feature is indicated on the α11 and α9 helices.A) Binary WT:NADP(H) 13 collapses to a closed conformation upon ICT binding, though moderate levels of deuterium exchange are still permitted.B) Binary R132Q:NADP(H) collapses to a closed conformation upon ICT binding, showing improved catalytic efficiency for the conventional reaction and lower deuterium uptake compared to R132H.However, catalytic activity is much lower compared to WT. C) Binary R132H:NADP(H) 29 collapses to a fully closed conformation only upon αKG binding 14 , but a seatbelt is not formed and deuterium uptake remains high.D) Binary R132Q:NADP(H) forms semi-closed and closed conformations upon binding αKG and NADP-αKG, respectively, with a seatbelt successfully formed in the closed state.The αKG binding site was shifted away from the α9 helix, though catalytic activity was much higher than that seen in R132H.

Discussion
Steady-state and pre-steady-state kinetic, HDX-MS, and X-ray crystallography experiments revealed fundamental differences in the molecular mechanisms of catalysis by WT and tumor-relevant IDH1 mutants (Fig. 8).It is unsurprising that IDH1 WT is far more efficient at catalyzing the conventional reaction than R132Q and R132H since R132 coordinates the C3 carboxylate of isocitrate 3,13 .As neither mutant can directly participate in this coordination, we asked why the conventional reaction was more efficient in R132Q than R132H.We found that R132Q employed a unique active site water that mitigated the loss of hydrogen bonding to ICT resulting from the R to Q mutation by imperfectly mimicking the polar interactions with the substrate normally afforded by R132 (Extended Data Fig. 9).Despite the shifting of the αKG binding site, we noticed a similar compensatory mechanism in our αKG-bound R132Q structure, with a water molecule recapitulating the polar interactions normally made by residue R132.Here, however, the water molecule did not appear to hydrogen bond with the substrate.Instead, a second water molecule was found at the same location as the Ca 2+ ion in the quaternary ICT-bound WT IDH1 structure (Extended Data Fig. 9), which presumably helped stabilize the αKG substrate in R132Q.We have previously reported the importance of water molecules in facilitating mutant IDH1 inhibition 30 , and this current work highlights the importance of water in substrate binding by providing a possible mechanism by which R132Q is more catalytically efficient compared to R132H.
In addition to affecting catalysis, the α10 regulatory segment may also serve as a selectivity filter for mutant IDH1 inhibitor binding 38 .We have shown previously that IDH1 R132Q binds poorly to selective mutant IDH1 inhibitors, with IC50 profiles consistent with IDH1 WT rather than R132H 22 .We had predicted that a much more stable α10 regulatory segment in R132Q:NADP(H) drove this resistance.Here, we found that while this unfolded loop indeed had stronger electron density compared to R132H:NADP(H) 29 , it still appeared less stable than the partially folded features of WT:NADP(H) 13 .Instead, we now believe that the more activated, quaternary-like state of the binary R132Q:NADP(H) complex helps drive inhibitor resistance.In the binary R132Q complex, regions including the α11 and α4 helices were shifted inwards and the protein experienced less deuterium uptake (Fig. 8).Using compound 24 as a prototypical selective mutant IDH1 inhibitor (6O2Y 39 ), it did not appear that the small increase in the stability of the α10 regulatory segment in IDH1 R132Q would have much of an effect on inhibitor binding (Extended Data Fig. 10).Instead, our alignments showed residues 111-121 in the inhibitor binding pocket, which form a loop between the β4 and β5 strands, likely have a larger role in the loss of affinity towards inhibitors for IDH1 R132Q.While this region accommodated the inhibitor in the R132H:NADP(H) complex, these residues would interfere with inhibitor binding to R132Q:NADP(H).
Interestingly, unlike in R132Q, these residues didn't appear to preclude inhibitor binding in WT:NADP(H).Thus, while it is the α10 regulatory segment that precludes inhibitor binding in IDH1 WT, it is instead residues 111-121 that prevent inhibitor binding in R132Q.This suggests that the essentially kinetically identical inhibitory characteristics of IDH1 WT and R132Q 22 develop through two very different mechanisms.Importantly, as this loop would not have been readily apparent as a selectivity gate when only examining the IDH1 WT structure, it is only through our R132Q:NADP(H) structure that we were able to identify a possible novel resistance strategy and selectivity handle.
While much effort has been devoted to understanding the unique catalytic and structural features of IDH1 WT versus R132H, our discovery of the unusual kinetic properties of the IDH1 R132Q mutant allowed a valuable opportunity to establish the static and dynamic structural adjustments required to maintain conventional and neomorphic activities within the same active site.Compared to IDH1 R132H, our findings show that the IDH1 R132Q binding pocket and surrounding areas are better primed for substrate binding and hydride transfer steps.Rather than simply acting as a hybrid of WT and R132H, IDH1 R132Q employed unique strategies to yield improved catalytic parameters for both ICT and αKG turnover as compared to R132H.These structural and dynamic discoveries not only highlight mechanistic properties of important tumor drivers, but also identify novel regions that may serve as selectivity handles when designing mutant IDH1 inhibitors requiring increasing selectivity or optimization against resistance mutants.
For pre-steady-state kinetics and HDX-MS experiments, protein was loaded onto a pre-equilibrated (50 mM Tris-HCl 7.5 at 4 °C and 100 mM sodium chloride) Superdex 16/600 size exclusion column (GE Life Sciences, Chicago, IL) following Ni-NTA affinity chromatography to remove any protein aggregate.Protein was eluted with 50 mM Tris-HCl pH 7.5 at 4 ºC, 100 mM NaCl, and 1 mM DTT.The fractions were pooled and concentrated for use in pre-steady-state experiments, or pooled and dialyzed in Tris-HCl pH 7.5 at 4 ºC, 100 mM NaCl, 20% glycerol, and 1 mM DTT and used immediately for HDX-MS analysis 31 .For IDH1 R132Q X-ray crystallography experiments, two 1 L cultures of terrific broth supplemented with 50 μg/ml of kanamycin were incubated at 37 °C and 180 rpm until an A600 of 0.4 was reached.Cultures were removed and placed onto stir plates and allowed to cool to 25 °C.Expression was induced when cultures reached an A600 of 0.8-1.0 with 1 mM IPTG and incubated for an additional 16-18 hours.Cell pellets were harvested and resuspended in lysis buffer (20 mM Tris pH 7.5 at 4 °C, 500 mM NaCl, 0.2% Triton X-100, 5 mM imidazole, 1 mM PMSF, and 5 mM BME).Following cell lysis via sonication, crude lysate was clarified via centrifugation at 14,000 x g for one hour.The lysate was loaded on to a pre-equilibrated Ni-NTA column.The column was washed with 100 mL of wash buffer (20 mM Tris pH 7.5 at 4 °C, 500 mM NaCl, 15 mM imidazole, 5 mM BME).Protein was eluted using elution buffer (50 mM Tris pH 7.5 at 4°C, 500 mM NaCl, 500 mM imidazole, 5% glycerol, 10 mM BME).
For the NADP(H)-stripped experiments, a buffer containing αKG was passed through the Ni-NTA affinity column prior to elution as described in previous work 14 .In all cases, eluted protein was loaded onto a HiPrep 26/10 desalting column (GE Healthcare) containing 25 mM Tris pH 7.5 at 20 °C, 500 mM NaCl, 5 mM EDTA, 2 mM DTT, and placed on ice overnight to remove any remaining metals from purification.Fractions containing IDH1 were concentrated (MilliPore Amicon Ultra 15 30 kDa NMWL concentrator) and loaded onto a Superdex 26/600 (GE Healthcare) pre-equilibrated with 20 mM Tris pH 7.5 at 20 °C, 200 mM NaCl, and 2 mM DTT.
Fractions containing pure IDH1 were pooled and concentrated to a final concentration of 14-20 mg/mL, flash frozen using liquid nitrogen, and stored at -80 °C.In all cases, the purity of the protein (>95% was confirmed using SDS-PAGE analysis).
Molecular graphics images.Structure figures were prepared using PyMOL 40 .
Kinetics assays.To measure steady-state activity of homodimer WT, R132H, and R132Q, only minor modifications were made from previous studies 21,22 .For the conventional reaction (ICT to αKG), IDH1 buffer (50 mM Tris HCl pH 7.5 at 37 °C, 150 mM NaCl, 10 mM MgCl2, 1 mM DTT) and homodimer IDH1 (100 nM IDH1 WT, or 200 nM IDH1 R132H and R132Q), as well as various concentrations of ICT and 200 µM NADP + were preincubated separately for 3 min at 37 °C.Following addition of substrates at 37 °C, the increase of absorbance at 340 nm due to production of NADPH was monitored using an Agilent Cary UV/Vis 3500 spectrophotometer (Santa Clara, CA).For the neomorphic reaction (αKG to D2HG), IDH1 buffer and homodimer mutant IDH1 (200 nM) as well as various concentrations of αKG at pH 7.5 and 200 µM NADPH were separately preincubated for 3 min at 37 °C.Following addition of substrates at 37 °C, the decrease of absorbance at 340 nm due to consumption of NADPH was monitored.The kinetic parameters, which were obtained using at least two unique protein preparations, were determined as described previously 21,22 .
For the reducing agent inhibition steady-state studies, the conventional reaction conditions described above were repeated except one of three reducing agents (DTT, TCEP, or BME) were added at varying concentrations during the pre-incubation step with the enzyme before substrates were added.Upon obtaining Michaelis-Menten plots at various reducing agent concentrations, the inverse of both kobs and substrate concentration were plotted in Lineweaver-Burk analysis.
Single-turnover, pre-steady-state kinetic assays were performed for the neomorphic reaction at 37 ⁰C using an RSM stopped-flow spectrophotometer (OLIS, Atlanta, GA).For the neomorphic reaction, hydride transfer (NADPH to NADP + conversion) was monitored as a change in fluorescence as a function of time via measuring the depletion of NADPH signal by exciting the sample at 340 nm and scanning the emission spectrum from 410 to 460 nm.Final concentrations after mixing were as follows: 40 µM IDH1 R132Q or R132H, 10 µM NADPH, 10 mM αKG (IDH1 R132H) or 0.5 mM αKG (IDH1 R132Q), 50 mM Tris-HCl (pH 7.5 at 37 ºC), 150 mM NaCl, 0.1 mM DTT, and 10 mM MgCl2.The change in fluorescence as a function of time was fit to a single exponential equation (Y = A0e -kt ) using Graphpad Prism to obtain kobs.For IDH1 R132H, a higher concentration of αKG (20 mM) was used since 1 mM αKG showed an initial lag.
Single turnover pre-steady-state kinetics were also performed for the conventional reaction at 37 ⁰C to obtain rate constants associated with steps after NADP + binding through hydride transfer using an RSM stopped-flow spectrophotometer.NADPH formation as a function of time was similarly monitored by exciting at 340 nm and scanning the emission spectrum from 410 to 460 nm.Final concentrations after mixing were as follows: 30 µM IDH1 WT or R132Q, 10 µM NADP + , 0.5 mM ICT (IDH1 WT) or 1 mM ICT (IDH1 R132Q), 50 mM Tris-HCl (pH 7.5 at 37 ºC), 150 mM NaCl, 0.1 mM DTT, and 10 mM MgCl2.The change in fluorescence as a function of time was fit to a single exponential equation (Y = A0e -kt ) using Graphpad Prism and kobs values were obtained.
Rates associated with NADPH binding corresponding to the first step of the catalytic cycle for the neomorphic reaction were performed as previously described 14 using an RSM-stopped flow spectrophotometer (OLIS, Atlanta, Georgia).However, due to low sensitivity of our stopped-flow spectrophotometer, the concentrations of NADPH and IDH1 were increased 10-fold, which in the case of IDH1 WT led to rates too fast to be detected by our instrument (≤100 s -1 ).Therefore, glycerol (40%) and temperature (10  For ICT titrations, experiments were performed at 25 ºC in 20 mM Tris pH 7.5, 100 mM NaCl, 10 mM CaCl2, and 2 mM BME, injecting 0.6mM ICT into the cell containing 0.12 mM IDH1 R132Q or 0.13 mM IDH1 R132H.
Baseline control experiments were performed by injecting the ligand into a cell with buffer only.In all cases, ITC data were analyzed using the Nanoanalyze software package by TA Instruments.
HDX-MS data collection and analysis.HDX-MS data collection and analysis was performed at the Biomolecular and Proteomics Mass Spectrometry Facility (BPMSF) of the University California San Diego using a Waters Synapt G2Si system with HDX technology (Waters Corporation, Milford, MA) as previously described, again using a sample of IDH1 WT without substrates to be analyzed alongside every experiment to allow experiment to experiment comparisons 31,41 .Deuterium exchange reactions were conducted using a Leap HDX PAL autosampler (Leap Technologies, Carrboro, NC).The D2O buffer was prepared by lyophilizing dialysis buffer (50 mM Tris buffer at pH 7.5 at 4 ºC, 100 mM NaCl, and 1 mM DTT) either alone (IDH1:NADP(H) condition) or with the following ligands: for the conventional reaction experiments, IDH1 WT and R132Q were treated with 0.01 mM NADP + and 10 mM ICT (ternary complexes), or with 0.1 mM NADP + , 10 mM ICT, and 10 mM CaCl2.For the neomorphic reaction experiments, IDH1 R132Q and R132H were treated with 0.1 mM NADPH and 10 mM αKG (ternary complexes), or with 0.1 mM NADPH, 10 mM αKG, and 10 mM CaCl2 was also included (quaternary complexes).The buffer was first prepared in ultrapure water and then redissolved in an equivalent volume of 99.96% D2O (Cambridge Isotope Laboratories, Inc., Andover, MA) just prior to use.Deuterium exchange measurements were performed in triplicate for every time point (in the order of 0 min, 0.5 min, 1 min, 2 min, 5 min); each run took 30 min to complete.Samples were prepared ~ 30 min prior to experimental setup and stored at 1 ºC until dispensing into reaction vials at the start of the reaction, resulting in samples that were exposed to its deuterium buffer between 2 h (0.5 min timepoint) and To identify peptides, data was collected on the mass spectrometer in mobility-enhanced dataindependent acquisition (MS E ), mobility ESI+ mode.Peptide masses were determined from triplicate analyses, and resulting data were analyzed using the ProteinLynx global server (PLGS) version 3.0 (Waters Corporation).We identified peptide masses using a minimum number of 250 ion counts for low energy peptides and 50 ion counts for their fragment ions, with the requirement that peptides had to be larger than 1,500 Da in all cases.Peptide sequence matches were filtered using the following cutoffs: minimum products per amino acid of 0.2, minimum score of 7, maximum MH+ error of 5 ppm, and a retention time RSD of 5%.To ensure high quality, we required that all peptides were present in two of the three experiments.After identifying peptides in PLGS, we then used DynamX 3.0 data analysis software (Waters Corporation) for peptide analysis.
Here, relative deuterium uptake for every peptide was calculated via comparison of the centroids of the mass envelopes of the deuterated samples with non-deuterated controls per previously reported methods 42 , and used to obtain data for coverage maps.Data are represented as mean values +/-SD of the three technical replicates due to processing software limitations, but we note that the LEAP robot provides highly reproducible data for biological replicates.Back-exchange was corrected for in the deuterium uptake values using a global back exchange correction factor (typically ~25%) determined from the average percent exchange measured in disordered termini of varied proteins 43 .Significance among differences in HDX data points was assessed using ANOVA analyses and t tests (p value cutoff of 0.05) within DECA 44 .We generated deuterium uptake plots in DECA (github.com/komiveslab/DECA), with data plotted as deuterium uptake (corrected) versus time.
An HDX-MS data summary table is shown in Supplementary Table 5, and percent uptake plots are shown in the Source Data files.
Crystallization.For the NADP(H)-only bound IDH1 R132Q crystals (PDB 8VHC, 8VH9), enzyme (14-20 mg/mL) was incubated on ice with 10 mM NADPH.Crystals of R132Q:NADP(H) were grown via hanging drop vapor diffusion at 4 °C. 2 μL of IDH1 were mixed with 2 μL of well solution containing either 220 mM ammonium sulfate, 100 mM bis-tris pH 6.5, and 20% (w/v) PEG 3350 (PDB ID 8VHC), or well solution containing 200 mM ammonium citrate tribasic pH 7.0 and 26% (w/v) PEG 3350 (8VH9).Though both forms aligned very well and appeared otherwise identical, we feared the citrate buffer could nonetheless promote more substrate-bound-like features due to its structural similarity to isocitrate.Thus, the binary structure crystallized in sulfate was used for all further comparisons and alignments.IDH1 R132Q crystals containing ICT (8VHD) were grown by first incubating the enzyme at 20 mg/mL with 10 mM NADP + , 10 mM CaCl2, and 200 mM DL-isocitric acid at 20°C for 1 h.Then, 2 µL of IDH1 were mixed with 2 μL of well solution containing 100 mM bis-tris propane pH 6.5, 200 mM NaI, and 24% (w/v) PEG 3350 and stored at 4 °C.Crystals were harvested using a nylon-loop and cryo-protected using a solution of 100 mM bis-tris propane pH 6.5, 200 mM NaI, 26%(w/v) PEG 3350, and 20%(v/v) glycerol.Crystals were flash-frozen in liquid nitrogen and stored until data collection.IDH1 R132Q crystals containing αKG and/or αKG-adducts were generated by incubating enzyme (14-20 mg/mL) on ice with 10 mM NADPH, 20 mM CaCl2, 75 mM αKG Fisher Scientific (Hampton, NH) for 1 h.For the 8VHB structure, crystals were grown at 4°C via hanging drop vapor diffusion, where 2 μL of IDH1 were mixed with 2 μL of the well solution containing 200 mM NaSCN and 21%(w/v) PEG 3350.Crystals were cryoprotected using a solution of 20% (v/v) glycerol, 25% (w/v) PEG 3350 and 200 mM NaSCN, and flash-frozen in liquid nitrogen and stored until data collection.For the 8VHA structure, IDH1 R132Q was incubated at 20 °C with 10 mM NADPH, 10 mM CaCl2, 10 mM αKG, and then crystals were grown at 4 °C by mixing 2 μL of IDH1 R132Q with 2 μL of well solution containing 160 mM NaNO3 and 20% (w/v) PEG 3350.Crystals were harvested using a nylon-loop and cryo-protected in a solution containing 22% (v/v) glycerol and 26% (w/v) PEG 3350.
Data collection, processing, and refinement.Data were collected at 100K using synchrotron radiation at the Advanced Photon Source, beamline 24-ID-E or at the Stanford Synchrotron Radiation Lightsource, beamline BL12-2.All datasets were processed with XDS 45 .Structure solutions were obtained by molecular replacement using PHASER-MR in Phenix 46,47 .For αKG and/or αKG-adducts (8VHB and 8VHA), isocitrate (8VHD), and NADP-TCEP (8VHE) co-crystals, PDB ensembles of 1T0L 13 , 4KZO 14 , and 6PAY 26 were used for molecular replacement by generating ensembles using Phenix Ensembler 46,47 .For IDH1 R132Q apo structures, 1T09 and 4UMX were used as search models.The models were optimized via iterative rounds of refinement in Phenix Refine and manual rebuilding in Coot 48,49 .Ligand restraints were generated in Phenix eLBOW 46,47 .Data collection and refinement statistics are summarized in Supplementary Table 6, and a stereo-image of the electron density maps for each new structure are shown in Supplementary Figure 6.
Calculations.Density functional theory (DFT) calculations 50 were carried out to model the NADP-TCEP binding energetics and geometry using the Gaussian 16 suite of programs 51 .The NADP + was modeled as the nicotinamide ring plus a pendant dihydroxy furan to represent the sugar.The model NADP + and NADP-TCEP adduct were each given a +1 charge.To better model the effects of the solvent, three explicit water molecules were included in calculations on the adducts, distributed at the likeliest sites for hydrogen bonding.The B3LYP 52 , ωB97XD 53 , and M06 54 hybrid functionals were used with the cc-pVDZ 55,56 and pc-n 57,58 basis sets, with the latter obtained from the online Basis Set Exchange 59 .In all of these calculations, implicit solvation was applied using the COSMO model with water as the solvent 60,61 and empirical dispersion was added using the D3 version of Grimme's dispersion along with Becke-Johnson damping 62,63 .This treatment of solvation effectively models the species as though they were in solution rather than crystalline form.Harmonic frequency analysis was carried out to obtain the vibrational corrections needed to calculate the free energies.Finally, because basis set superposition error can be substantial relative to intermolecular bond energies, the counterpoise correction was applied to our final energies of reaction 64,65 .The transition state (TS) for the TCEP + NADP binding was identified and confirmed by analysis of the single imaginary vibrational frequency.
The DFT calculations for the model NADP-TCEP adduct predicted values of 25º for Δ ! and -11º for Δ " , where the experimental values in the X-ray structure were Δ != 29.2ºand Δ " = -1.1º(Supplementary Table 2).For the NADP-αKG adduct, agreement was similar, with DFT predicting Δ !=29º and Δ " = -14º as compared to Δ !=25º and Δ " = -25º in the X-ray structure (Supplementary Table 2).The binding was energetically favored, and appeared to occur without barrier when vibrational effects were included, with a calculated binding energy of 9.4 kcal mol -1 at 298 K.However, the calculated free energies indicated that in solution, the entropy decrease would preclude spontaneous binding.Quenching the translational entropy of the species in the crystal may be what allowed the process to occur.We noted that the counterpoise corrections to the transition state and adduct energies were essential, having magnitudes of 7-8 kcal mol -1 and comparable to the uncorrected energy differences.
For the dihedral angles, the deviation from planarity Δ of the NADP pyridine ring in the adduct was reported using the average of two dihedral angles.Numbering the carbon atoms in the ring by convention as shown in Appendix Fig S2, the C-P bond in NADP-TCEP formed at atom 4. The positions of the N atom 1 and the opposite C atom 4 are referenced to the plane defined by the roughly coplanar atoms 2, 3, 5, and 6.The average of the dihedral angles 2-3-5-4 and 6-3-5-4 (Supplementary Fig. 5) was subtracted from 180 o to yield Δ ! as a metric for the deviation from planarity of C4, while the average of 3-2-6-1 and 5-2-6-1 subtracted from 180 o is used to calculate Δ " for N1.A sign convention was applied such that if Δ ! and Δ " had the same sign, the two corners of the ring bend away each other in chair fashion, whereas opposite signs indicate a boat-like conformation.Comparison of the results from the different functionals and basis sets showed very little difference in the geometry.Optimized geometries obtained with the pc-2 basis set on a smaller geometry (omitting sugar and explicit waters) were not significantly different from those obtained with pc-1, so we chose to report the B3LYP/pc-1 results here, with the sugar and explicit waters included (Supplementary Table 2).An additional geometry optimization was run on the NADP-αKG adduct with two explicit waters and a -2 charge, employing the aug-pc-1 basis set 57,66 to obtain the diffuse functions necessary to adequately model anions.

Fig. 1 .
Fig. 1.Pre-steady-state single-turnover kinetic features of IDH1 WT, R132H, and R132Q catalysis.NADPH formation in the conventional reaction and consumption in the neomorphic reaction was monitored over the course of a single turnover (top plot) and compared with a control experiment lacking enzyme (bottom plot, in green).Residuals (middle plot) were obtained to assess goodness of a single exponential equation fit.A) IDH1 WT, conventional reaction.B) IDH1 R132H, neomorphic reaction.C) IDH1 R132Q, conventional reaction.D) IDH1 R132Q, neomorphic reaction.

Fig. 6 .
Fig. 6.Deuterium uptake by IDH1 WT, R132Q, and R132H in helices bounding the substrate binding pocket.Deuterium uptake is shown as a gradient from red (high uptake) to blue (low uptake).A) Deuterium uptake by IDH1 WT, R132Q, and R132H upon no ligand treatment.These HDX-MS data were overlaid on NADP(H)-only bound forms of WT 13 in all three cases, as the αKG helix was disordered in the NADP(H)-only bound forms of IDH1 R132Q and R132H 29 .B) Deuterium uptake by WT and R132Q upon treatment with NADP + and ICT, and by IDH1 R132Q and R132H upon treatment with NADPH and αKG.These HDX-MS data were overlaid on WT:NADP(H):ICT:Ca 2+ 13 , R132Q:NADP(H):ICT:Ca 2+ and R132Q:NADP(H):αKG:Ca 2+ , or R132H:NADP(H): αKG:Ca 2+ 14 .C) Deuterium uptake by IDH1 WT and R132Q upon treatment with NADP + , ICT, and Ca 2+ , and by IDH1 R132Q and R132H upon treatment with NADPH, αKG, and Ca 2+ .These HDX-MS data were overlaid on the structures described in (B).
ºC) were used to slow NADPH binding rates to IDH1.NADPH binding as a function of time was monitored by exciting at 340 nm and scanning the emission spectrum from 410 to 460 nm.Final concentrations after mixing were as follows: 4 µM IDH1, varying concentration of µM NADP + , 100 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1 mM DTT, 10 mM MgCl2, and 40% glycerol.The change in fluorescence as a function of time was fit to a single exponential equation (Y = A0e -kt ) using Graphpad Prism, and kobs values were obtained and plotted as a function of NADPH concentration using the equation kobs = k1[NADPH] + k-1.This yielded a linear graph indicating one-step binding, with the slope equal to k1 and the Y-intercept equal to k-1, though the Y-intercept slope was too high to do so reliably.Isothermal titration calorimetry (ITC) experiments were conducted at the Sanford Burnham Prebys Protein Production and Analysis Facility using a Low Volume Affinity ITC calorimeter (TA Instruments).For NADPH titrations, experiments were performed at 25 ºC in 20 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2, and 2 mM BME, injecting 0.25 mM NADPH into the cell containing 0.025 mM for IDH1 WT, 0.025 mM or 0.04 mM IDH1 R132H; and injecting 0.15 mM NADPH into the cell containing 0.034 mM or 0.026 mM IDH1 R132Q.